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indispin® pathogen kit (INDICAL BIOSCIENCE)

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INDICAL BIOSCIENCE indispin® pathogen kit
Indispin® Pathogen Kit, supplied by INDICAL BIOSCIENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/indispin+pathogen+kit/indispin+pathogen+kit/pm40527404-62-18-21
Average 90 stars, based on 1 article reviews

indispin® pathogen kit - by Bioz Stars, 2026-09

90/100 stars

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Synthesized:

Article Title: An African swine fever vaccine-like variant with multiple gene deletions caused reproductive failure in a Vietnamese breeding herd
Article Snippet: .. In the laboratory, whole blood or tissue homogenates were extracted for viral DNA/RNA using the IndiSpin Pathogen Kit (Indical Bioscience GmbH, Leipzig, Germany), and complementary DNA (cDNA) was synthesized with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) following manufacturer’s instruction. .. Nucleic acid quantity and quality were assessed using Epoch2NS ® Microplate Spectrophotometer Reader and Gen5TM Microplate Software (Agilent Technologies Inc., Santa Clara, CA, USA) by measuring the A260/280 ratio.

cDNA Synthesis:

Lysis:

Article Title: The cervical microbiome of ewe breeds with known divergent fertility following artificial insemination with frozen-thawed semen
Article Snippet: .. DNA was extracted using the IndiSpin Pathogen Kit (Indical Biosciences GmbH, Leipzig, Germany) with the following modifications: the tubes containing the cytobrushes were centrifuged at maximum speed in a tabletop centrifuge, and the resultant pellet was dissolved in 500 μl Buffer ATL and transferred to a Pathogen Lysis Tube S (Qiagen, Hilden, Germany) together with the cytobrush for mechanical lysis on a TissueLyser II (Qiagen) for 10 min at a frequency of 30 Hz. ..

DNA Extraction:

Article Title: Presence of Francisella tularensis in Apodemus spp. on the Edges of Forest Areas in Belgrade, Serbia.
Article Snippet: Tularemia, a zoonosis caused by Francisella tularensis, is considered endemic to the Balkan Peninsula.. The main reservoirs of this disease are rodents and lagomorphs, which usually show no clinical signs and are a potential disease source for other animals and humans.. The presence of F. tularensis in the tissues of rodents in Serbia was examined for the first time.

Real-time Polymerase Chain Reaction:

Article Title: National survey of the rat hepatitis E virus in rodents in Spain, 2022 to 2023
Article Snippet: .. Therefore, viral RNA was evaluated following the same qPCR protocol as described above, but the RNA was extracted using the IndiSpin Pathogen Kit (Indical Bioscience, Leipzig, Germany). ..

Quantitative RT-PCR:

Article Title: Occurrence and genetic diversity of the zoonotic rat hepatitis E virus in small mammal species, Spain.
Article Snippet: .. For that, viral RNA was evaluated following the same RT-qPCR protocol described above but extracting the RNA using the IndiSpin Pathogen Kit (Indical Bioscience). ..